Infrastructure / Equipments



Confocal Laser Scanning Microscope

Peptide Synthesis Facility

Amino Acid Analysis Facility

 


Confocal
Laser Scanning Microscope

Confocal microscopy offers several advantages over conventional optical microscopy, including controllable depth of field, the elimination of image degrading out-of-focus information, and the ability to collect serial optical sections from thick specimens.

The Institute has a Confocal Laser Scanning Microscope (LSM) with spectral imaging. The system is mounted on an inverted microscope with temperature controlled stage. The laser systems include the Argon and Helium-neon lasers for fluorescence excitation of 458/488, 477, 514 and 633 nm, the DPSS and blue diode laser for 561, 405 nm excitation wavelengths. The scanning module has two single-channel detectors and polychromatic multichannel detector capable of simultaneous acquisition of up to 8 channels. Beside the feature of high resolution optical slicing and 3 dimensional imaging of the cells and tissues, it can also perform FRAP, FRET, FLIM and live cell imaging studies.

Investigators perform the labeling/staining as per requirements using appropriate flourophores in their laboratory. The system is available for viewing and analysis. Presently we have the expertise in co-localization and 3D reconstruction of the samples. Future plans are to include FRET and FRAP analysis as well.

Contact Person: Dr. Nafisa Balasinor (Telephone No: 91-22-24192025)

Confocal Laser Scanning Microscope