Our department is focused extensively towards elucidating the pathogenic mechanisms underlying the development of the polycystic ovary syndrome (PCOS) using a multi-pronged approach consisting of genetic, proteomic and epigenetic methods.
Genetic studies are being carried out using candidate gene approach to establish a predisposing genetic profile of Indian women with PCOS. Our data establishes that in Indian women, variations in genes related to insulin resistance influence the pathophysiology of PCOS and its related traits. We are currently working towards developing a more comprehensive genetic predisposition profile for PCOS. We are also currently engaged in improving the current understanding on epigenetic involvement in PCOS.
Our group has recently generated a protein dataset of follicular fluid wherein the largest number of proteins (770 proteins) has been reported till date. Many of these proteins are novel and have not been reported in ovary previously. We have compared the follicular fluid of normal versus PCOS ovary using iTRAQ based quantitative proteomics followed by high resolution LC-MS/MS. Presently we are investigating the role of several of these proteins in folliculogenesis and PCOS pathogenesis which may help predict biomarkers for determining oocyte quality and IVF outcomes.
Our department has also developed an animal model to understand the cellular and molecular basis of cyst formation in ovaries. Epigenetic modifications have been proven to be equally influential in the regulation of complex diseases.
The department is well equipped with advanced facilities for experimentation including DNA sequencers, flow cytometer and thermal cyclers.
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